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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.6
- 수록면
- 153 - 157 (5page)
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Although culture is the gold standard method to identify mycobacteria, its use in tuberculous lymphadenitis (TBL) is limited due to formalin fixation of the submitted specimens. We evaluated the performance of quantitative real-time PCR (q-PCR) for Mycobacterium Tuberculosis (MTB) in granulomatous lymphadenitis using formalin-fixed paraffinembedded (FFPE) tissues. From 2000 to 2010, a total number of 117 cases of lymph node samples with granulomatous inflammation which were surgically removed and fixed in formalin were studied. Hematoxylin & Eosin (H&E) and Ziehl-Neelsen-stained (ZN) slides were reviewed. qPCR using Real TB-Taq<SUP>®</SUP> was performed for all cases to identify Mycobacterium tuberculosis. Thirteen non-tuberculous lymphadenopathy cases were used as negative control. Cervical lymph nodes were more frequently affected (60%, 70/117) than other sites. ZN stain for acid fast bacilli was positive in 19 (16.24%) cases. qPCR for tuberculosis was positive in 92 (78.63%) cases. Caseous necrosis was found in 103 (88.03%) cases. While the ZN stain and qPCR were both negative in all control cases, the qPCR showed a significantly higher positive rate (78.63% vs. 16.24%) compared to ZN stain in histologically diagnosed TBL. Quantitative real-time PCR proves to be more sensitive than ZN stain for diagnosis of tuberculous lymphadenitis.
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참고문헌 (19)
참고문헌 신청
Ahmed HG / 2011 / Screening for tuberculosis and its histological pattern in patients with enlarged lymph node / Patholog Res Int 2011:417635
Bruijnesteijn Van Coppenraet ES / 2004 / Real-time PCR assay using fine-needle aspirates and tissue biopsy specimens for rapid diagnosis of mycobacterial lymphadenitis in children / J Clin Microbiol 42:2644~2650
Causse M / 2011 / Comparison of two molecular methods for rapid diagnosis of extrapulmonary tuberculosis / J Clin Microbiol 49:3065~3067
Daley P / 2007 / Nucleic acid amplification tests for the diagnosis of tuberculous lymphadenitis: a systematic review / Int J Tuberc Lung Dis 11:1166~1176
Greco S / 2006 / Current evidence on diagnostic accuracy of commercially based nucleic acid amplification tests for the diagnosis of pulmonary tuberculosis / Thorax 61:783~790
Bruijnesteijn Van Coppenraet ES / 2004 / Real-time PCR assay using fine-needle aspirates and tissue biopsy specimens for rapid diagnosis of mycobacterial lymphadenitis in children / J Clin Microbiol 42:2644~2650
Causse M / 2011 / Comparison of two molecular methods for rapid diagnosis of extrapulmonary tuberculosis / J Clin Microbiol 49:3065~3067
Daley P / 2007 / Nucleic acid amplification tests for the diagnosis of tuberculous lymphadenitis: a systematic review / Int J Tuberc Lung Dis 11:1166~1176
Greco S / 2006 / Current evidence on diagnostic accuracy of commercially based nucleic acid amplification tests for the diagnosis of pulmonary tuberculosis / Thorax 61:783~790
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UCI(KEPA) : I410-ECN-0101-2014-500-003262973