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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.4
- 수록면
- 176 - 184 (9page)
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Purpose: Protein overloading in the endoplasmic reticulum (ER) leads to endoplasmic reticulum stress, which exacerbates various disease conditions. Emodin, an anthraquinone compound, is known to have several health benefits. The effect of emodin against palmitic acid (PA) - induced ER stress in HepG2 cells was investigated. Methods: HepG2 cells were treated with varying concentrations of palmitic acid to determine the working concentration that induced ER stress. ER stress associated genes such as ATF4, XBP1s, CHOP and GRP78 were checked using RT- PCR. In addition, the expression levels of unfolded protein response (UPR) associated proteins such as IRE1α, eIF2α and CHOP were checked using immunoblotting to confirm the induction of ER stress. The effect of emodin on ER stress was analyzed by treating HepG2 cells with 750 μM palmitic acid and varying concentrations of emodin, then analyzing the expression of UPR associated genes. Results: It was evident from the mRNA and protein expression results that palmitic acid significantly increased the expression of UPR associated genes and thereby induced ER stress. Subsequent treatment with emodin reduced the mRNA expression of ATF4, GRP78, and XBP1s. Furthermore, the protein levels of p-IRE1α, p-elF2α and CHOP were also reduced by the treatment of emodin. Analysis of sirtuin mRNA expression showed that emodin increased the levels of SIRT4 and SIRT7, indicating a possible role in decreasing the expression of UPR-related genes. Conclusion: Altogether, the results suggest that emodin could exert a protective effect against fatty acid-induced ER stress and could be an agent for the management of various ER stress related diseases.
목차
ABSTRACT
Introduction
Methods
Results
Discussion
Summary
References
참고문헌 (30)
참고문헌 신청
Zhao L / 2006 / Endoplasmic reticulum stress in health and disease / Curr Opin Cell Biol 18(4):444~452
Koga T / 2015 / Endoplasmic reticulum(ER)stress induces Sirtuin 1(SIRT1)expression via the PI3K-Akt-GSK3β signaling pathway and promotes hepatocellular injury / J Biol Chem 290(51):30366~30374
Regina M Graham / 2016 / Resveratrol augments ER stress and the cytotoxic effects of glycolytic inhibition in neuroblastoma by downregulating Akt in a mechanism independent of SIRT1 / Experimental and Molecular Medicine 48:1~12
Zhang K / 2005 / The unfolded protein response sensor IRE1alpha is required at 2 distinct steps in B cell lymphopoiesis / J Clin Invest 115(2):268~281
Miyagawa K / 2016 / Lipid-induced endoplasmic reticulum stress impairs selective autophagy at the step of autophagosome-lysosome fusion in hepatocytes / Am J Pathol 186(7):1861~1873
Koga T / 2015 / Endoplasmic reticulum(ER)stress induces Sirtuin 1(SIRT1)expression via the PI3K-Akt-GSK3β signaling pathway and promotes hepatocellular injury / J Biol Chem 290(51):30366~30374
Regina M Graham / 2016 / Resveratrol augments ER stress and the cytotoxic effects of glycolytic inhibition in neuroblastoma by downregulating Akt in a mechanism independent of SIRT1 / Experimental and Molecular Medicine 48:1~12
Zhang K / 2005 / The unfolded protein response sensor IRE1alpha is required at 2 distinct steps in B cell lymphopoiesis / J Clin Invest 115(2):268~281
Miyagawa K / 2016 / Lipid-induced endoplasmic reticulum stress impairs selective autophagy at the step of autophagosome-lysosome fusion in hepatocytes / Am J Pathol 186(7):1861~1873
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UCI(KEPA) : I410-ECN-0101-2019-594-000768154