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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제17권 제6호
- 발행연도
- 2007.1
- 수록면
- 945 - 951 (7page)
이용수
초록· 키워드
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DNA-DNA hybridization has been established asan important technology in bacterial species taxonomy andphylogenetic analysis. In this study, we analyzed how theefficiency with which the genomic DNA from one specieshybridizes to the genomic DNA of another species (DNA-DNA hybridization) in microarray analysis relates to theDNA-DNA hybridization based on genome sequence similaritycorrelated well with the experimentally determined microarrayhybridization. Between closely related strains, significantnumbers of highly divergent genes (<55% identity) and/or theaccumulation of mismatches between conserved genes loweredthe DNA-DNA hybridization signal, and this reduced thehybridization signals to below 70% for even bacterial strainswith over 97% 16S rRNA gene identity. In adition, ourresults also suggest that a DNA-DNA hybridization signalintensity of over 40% indicates that two genomes at least sharedexpand our knowledge of DNA-DNA hybridization based ongenomic sequence similarity comparison and further provideinsights for bacterial phylogeny analyses.
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참고문헌 (27)
참고문헌 신청
A / 1980 / Reproducibility and correlation study of three deoxyribonucleic acid hybridization procedures : 325 ~ 330
Bae, J. W / 2005 / Development and evaluation of genome-probing microarrays for monitoring lactic acid bacteria 71 : 8825 ~ 8835
Brenner / 1972 / Polynucleotide sequence divergence among strains of Escherichia coli and closely related organisms : 953 ~ 965
Brenner / 1973 / Molecular relationships among the Salmonelleae : 307 ~ 315
Dorris, D. R / 2002 / A highly reproducible, linear, and automated sample preparation method for DNA microarrays 12 : 976 ~ 984
Bae, J. W / 2005 / Development and evaluation of genome-probing microarrays for monitoring lactic acid bacteria 71 : 8825 ~ 8835
Brenner / 1972 / Polynucleotide sequence divergence among strains of Escherichia coli and closely related organisms : 953 ~ 965
Brenner / 1973 / Molecular relationships among the Salmonelleae : 307 ~ 315
Dorris, D. R / 2002 / A highly reproducible, linear, and automated sample preparation method for DNA microarrays 12 : 976 ~ 984
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