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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제26권 제5호
- 발행연도
- 2016.1
- 수록면
- 846 - 853 (8page)
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초록· 키워드
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We displayed four types of Solanum nigrum metallothionein (SMT) for the first time on the surface of Saccharomyces cerevisiae using an α-agglutinin-based display system. The SMT genes were amplified by RT-PCR. The plasmid pYES2 was used to construct the expression vector. Transformed yeast strains were confirmed by PCR amplification and custom sequencing. Surface-expressed metallothioneins were indirectly indicated by the enhanced cadmium sorption capacity. Flame atomic absorption spectrophotometry was used to examine the concentration of Cd2+ in this study. The transformed yeast strains showed much higher resistance ability to Cd2+ compared with the control. Strikingly, their Cd2+ accumulation was almost twice as much as that of the wild-type yeast cells. Furthermore, surface-engineered yeast strains could effectively adsorb ultra-trace cadmium and accumulate Cd2+ under a wide range of pH levels, from 3 to 7, without disturbing the Cu2+ and Hg2+. Four types of surfaceengineered Saccharomyces cerevisiae strains were constructed and they could be used to purify Cd2+-contaminated water and adsorb ultra-trace cadmium effectively. The surface-engineered Saccharomyces cerevisiae strains would be useful tools for the bioremediation and biosorption of environmental cadmium contaminants.
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참고문헌 (31)
참고문헌 신청
Akbal F / 2012 / Treatment of metal plating wastewater by electrocoagulation / Environ. Prog. Sustain. Energy 31:340~350
Al exander J / 2009 / Scientific opinion of the panel on contaminants in the food chain on a request from the European Commission on cadmium in food / EFSA J 980:1~139
Arief VO / 2008 / Recent progress on biosorption of heavy metals from liquids using low cost biosorbents: characterization, biosorption parameters and mechanism studies / Clean (Weinh) 36:937~962
Bae W / 2000 / Enhanced bioaccumulation of heavy metals by bacterial cells displaying synthetic phytochelatins / Biotechnol. Bioeng 70:518~524
Batayneh AT / 2012 / Toxic (aluminum, beryllium, boron, chromium and zinc) in groundwater: health risk assessment / Int. J. Environ. Sci. Technol 9:153~162
Al exander J / 2009 / Scientific opinion of the panel on contaminants in the food chain on a request from the European Commission on cadmium in food / EFSA J 980:1~139
Arief VO / 2008 / Recent progress on biosorption of heavy metals from liquids using low cost biosorbents: characterization, biosorption parameters and mechanism studies / Clean (Weinh) 36:937~962
Bae W / 2000 / Enhanced bioaccumulation of heavy metals by bacterial cells displaying synthetic phytochelatins / Biotechnol. Bioeng 70:518~524
Batayneh AT / 2012 / Toxic (aluminum, beryllium, boron, chromium and zinc) in groundwater: health risk assessment / Int. J. Environ. Sci. Technol 9:153~162
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