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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제20권 제12호
- 발행연도
- 2010.1
- 수록면
- 1,630 - 1,636 (7page)
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During the fermentation process of Saccharomyces cerevisiae, yeast cells must rapidly respond to a wide variety of external stresses in order to survive the constantly changing environment, including ethanol stress. The accumulation of ethanol can severely inhibit cell growth activity and productivity. Thus, the response to changing ethanol concentrations is one of the most important stress reactions in S. cerevisiae and worthy of thorough investigation. Therefore, this study examined the relationship between ethanol tolerance in S. cerevisiae and a unique protein called alcohol sensitive RING/PHD finger 1 protein (Asr1p). A real-time PCR showed that upon exposure to 8% ethanol, the expression of Asr1was continuously enhanced, reaching a peak 2 h after stimulation. This result was confirmed by monitoring the fluorescence levels using a strain with a green fluorescent protein tagged to the C-terminal of Asr1p. The fluorescent microscopy also revealed a change in the subcellular localization before and after stimulation. Furthermore,the disruption of the Asr1 gene resulted in hypersensitivity on the medium containing ethanol, when compared with the wild-type strain. Thus, when taken together, the present results suggest that Asr1 is involved in the response to ethanol stress in the yeast S. cerevisiae.
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참고문헌 (24)
참고문헌 신청
Alexandre, H / 2001 / Global gene expression during short-term ethanol stress in Saccharomyces cerevisiae / FEBS Lett 498 : 98 ~ 103
Aravind, L / 2003 / Scores of RINGS but no PHDs in ubiquitin signaling / Cell Cycle 2 : 123 ~ 126
Bahler, J / 1998 / Heterologous modules for efficient and versatile PCRbased gene targeting in Schizosaccharomyces pombe / Yeast 14 : 943 ~ 951
Bailer, S. M / 2000 / Nup116p associates with the Nup82p-Nsp1p-Nup159p nucleoporin complex / J. Biol. Chem 275 : 23540 ~ 23548
Betz, C / 2004 / Asr1p, a novel yeast RING/PHD finger protein, signals alcohol stress to the nucleus / J. Biol. Chem 279 : 28174 ~ 28181
Aravind, L / 2003 / Scores of RINGS but no PHDs in ubiquitin signaling / Cell Cycle 2 : 123 ~ 126
Bahler, J / 1998 / Heterologous modules for efficient and versatile PCRbased gene targeting in Schizosaccharomyces pombe / Yeast 14 : 943 ~ 951
Bailer, S. M / 2000 / Nup116p associates with the Nup82p-Nsp1p-Nup159p nucleoporin complex / J. Biol. Chem 275 : 23540 ~ 23548
Betz, C / 2004 / Asr1p, a novel yeast RING/PHD finger protein, signals alcohol stress to the nucleus / J. Biol. Chem 279 : 28174 ~ 28181
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