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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제10호
- 발행연도
- 2015.1
- 수록면
- 1,614 - 1,620 (7page)
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초록· 키워드
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Plasmid-cured derivative strains of Bacillus anthracis are frequently used in laboratory studies. Plasmid incompatibility, which does not increase the risk of chromosomal mutation, is a useful method for plasmid curing. However, in bacteria containing multiple plasmids, it often requires the sequential introduction of multiple, specific incompatibility plasmids. This lengthy process renders the traditional plasmid incompatibility method inefficient and mutation-prone. In this study, we successfully cured plasmids pXO1 and pXO2 from B. anthracis A16 simultaneously using only one recombinant incompatible plasmid, pKORT, to obtain a plasmid-free strain, designated A16DD. This method may also be useful for the simultaneous, one-step curing of multiple plasmids from other bacteria, including Bacillus thuringiensis and Yersinia pestis.
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참고문헌 (11)
참고문헌 신청
Green BD / 1984 / Demonstration of a capsule plasmid in Bacillus anthracis / Infect. Immun 49 : 291 ~ 297
Kittell BL / 1993 / Plasmid incompatibility and replication control / Springer : 223 ~ 242
Liu X / 2012 / Curing of plasmid pXO1 from Bacillus anthracis using plasmid incompatibility / PLoS ONE 7 : 230 ~ 230
Novick RP / 1987 / Plasmid incompatibility / Microbiol. Rev 51 : 381 ~ 395
Park SH / 2007 / Dif ferential analysis of Bacillus anthracis a f ter pX01 plasmid curing and comprehensive data on Bacillus anthracis infection in macrophages and glial cell
Kittell BL / 1993 / Plasmid incompatibility and replication control / Springer : 223 ~ 242
Liu X / 2012 / Curing of plasmid pXO1 from Bacillus anthracis using plasmid incompatibility / PLoS ONE 7 : 230 ~ 230
Novick RP / 1987 / Plasmid incompatibility / Microbiol. Rev 51 : 381 ~ 395
Park SH / 2007 / Dif ferential analysis of Bacillus anthracis a f ter pX01 plasmid curing and comprehensive data on Bacillus anthracis infection in macrophages and glial cell
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