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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 356 - 362 (7page)
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The binding of MS2 bacteriophage coat protein (MCP) to MS2 binding site (MBS) RNA stem-loop sequences has been widely used to label mRNA for live-cell imaging at singlemolecule resolution. However, concerns have been raised recently from studies with budding yeast showing aberrant mRNA metabolism following the MS2-GFP labeling. To investigate the degradation pattern of MS2-GFP-labeled mRNA in mammalian cells and tissues, we used Northern blot analysis of β-actin mRNA extracted from the Actb-MBS knock-in and MBS×MCP hybrid mouse models. In the immortalized mouse embryonic cell lines and various organ tissues derived from the mouse models, we found no noticeable accumulation of decay products of β-actin mRNA compared with the wild-type mice. Our results suggest that accumulation of MBS RNA decay fragments does not always happen depending on the mRNA species and the model organisms used.
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참고문헌 (6)
참고문헌 신청
Tutucci, E. / 2018 / An improved MS2 system for accurate reporting of the mRNA life cycle / Nat. Methods 15:81~89
Vera, M. / 2016 / Single-cell and single-molecule analysis of gene expression regulation / Annu. Rev. Genet 50:267~291
Wang, Y. / 2002 / Precision and functional specificity in mRNA decay / Proc. Natl. Acad. Sci. USA 99:5860~5865
Wu, B. / 2015 / Quantifying protein-mRNA interactions in single live cells / Cell 162:211~220
Yang, E. / 2003 / Decay rates of human mRNAs : correlation with functional characteristics and sequence attributes / Genome Res 13:1863~1872
Vera, M. / 2016 / Single-cell and single-molecule analysis of gene expression regulation / Annu. Rev. Genet 50:267~291
Wang, Y. / 2002 / Precision and functional specificity in mRNA decay / Proc. Natl. Acad. Sci. USA 99:5860~5865
Wu, B. / 2015 / Quantifying protein-mRNA interactions in single live cells / Cell 162:211~220
Yang, E. / 2003 / Decay rates of human mRNAs : correlation with functional characteristics and sequence attributes / Genome Res 13:1863~1872
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