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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 279 - 286 (8page)
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Recent investigations consider adipose-derived stemcells (ASCs) as a promising source of stemcells for clinical therapies. To obtain functional cells with enhanced cytoskeleton and aligned structure, mechanical stimuli are utilized during differentiation of stem cells to the target cells. Since function of muscle cells is associated with cytoskeleton, enhanced structure is especially essential for these cells when employed in tissue engineering. In this study by utilizing a custom-made device, effects of uniaxial tension (1Hz, 10% stretch) on cytoskeleton, cell alignment, cell elastic properties, and expression of smooth muscle cell (SMC) genes in ASCs are investigated.Due to proper availability ofASCs, results can be employed in cardiovascular engineeringwhen production of functional SMCs in arterial reconstruction is required. Results demonstrated that cells were oriented after 24 hours of cyclic stretch with aligned pseudo-podia. Staining of actin filaments confirmed enhanced polymerization and alignment of stress fibers. Such phenomenon resulted in stiffening of cell body which was quantified by atomic force microscopy (AFM). Expression of SM a-actin and SM22 aactin as SMC associated genes were increased after cyclic stretch while GAPDH was considered as internal control gene. Finally, it was concluded that application of cyclic stretch on ASCs assists differentiation to SMC and enhances functionality of cells.
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참고문헌 (43)
참고문헌 신청
Ren G / 2012 / Concise review : mesenchymal stem cells and translational medicine : emerging issues / Stem Cells Transl Med 1:51~58
Mizuno H / 2012 / Concise review : adipose-derived stem cells as a novel tool for future regenerative medicine / Stem cells 30:804~810
Colazzo F / 2011 / Extracellular matrix production by adipose-derived stem cells : implications for heart valve tissue engineering / Biomaterials 32:119~127
Lindroos B / 2011 / The potential of adipose stem cells in regenerative medicine / Stem Cell Rev Rep 7:269~291
Kshitiz / 2012 / Control of stem cell fate and function by engineering physical microenvironments / Integr Biol(Camb) 4:1008~1018
Mizuno H / 2012 / Concise review : adipose-derived stem cells as a novel tool for future regenerative medicine / Stem cells 30:804~810
Colazzo F / 2011 / Extracellular matrix production by adipose-derived stem cells : implications for heart valve tissue engineering / Biomaterials 32:119~127
Lindroos B / 2011 / The potential of adipose stem cells in regenerative medicine / Stem Cell Rev Rep 7:269~291
Kshitiz / 2012 / Control of stem cell fate and function by engineering physical microenvironments / Integr Biol(Camb) 4:1008~1018
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