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논문 기본 정보

자료유형
학술저널
저자정보
Abo-Amer Aly E. (Department of Botany and Microbiology Faculty of Science Sohag University Sohag 82524 Egypt) Gad El-Rab Sanaa M. F. (Department of Botany and microbiology Faculty of Science Assuit University Assiut 71516 Egypt) Halawani Eman M. (Department of Biology College of Science Taif University P.O. Box 11099 Taif 21944 Saudi Arabia) Niaz Ameen M. (Department of Biology College of Science Taif University P.O. Box 11099 Taif 21944 Saudi Arabia) Bamaga Mohammed S. (Department of Molecular Pathology Al-Hada Armed Forces Hospital P.O. Box 1347 HHRC 479 Taif Saudi A)
저널정보
한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제32권 제12호
발행연도
2022.12
수록면
1,537 - 1,546 (10page)
DOI
10.4014/jmb.2208.08004

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Staphylococcus aureus is a cause of high mortality in humans and therefore it is necessary to prevent its transmission and reduce infections. Our goals in this research were to investigate the frequency of methicillin-resistant S. aureus (MRSA) in Taif, Saudi Arabia, and assess the relationship between the phenotypic antimicrobial sensitivity patterns and the genes responsible for resistance. In addition, we examined the antimicrobial efficiency and application of silver nanoparticles (AgNPs) against MRSA isolates. Seventy-two nasal swabs were taken from patients; MRSA was cultivated on Mannitol Salt Agar supplemented with methicillin, and 16S rRNA sequencing was conducted in addition to morphological and biochemical identification. Specific resistance genes such as ermAC, aacA-aphD, tetKM, vatABC and mecA were PCR-amplified and resistance plasmids were also investigated. The MRSA incidence was ~49 % among the 72 S. aureus isolates and all MRSA strains were resistant to oxacillin, penicillin, and cefoxitin. However, vancomycin, linezolid, teicoplanin, mupirocin, and rifampicin were effective against 100% of MRSA strains. About 61% of MRSA strains exhibited multidrug resistance and were resistant to 3-12 antimicrobial medications (MDR). Methicillin resistance gene mecA was presented in all MDR-MRSA strains. Most MDR-MRSA contained a plasmid of > 10 kb. To overcome bacterial resistance, AgNPs were applied and displayed high antimicrobial activity and synergistic effect with penicillin. Our findings may help establish programs to control bacterial spread in communities as AgNPs appeared to exert a synergistic effect with penicillin to control bacterial resistance.

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