A class I histone deacetylase HDA-2 is essential for embryonic development and size regulation of fertilized eggs in Caenorhabditis elegans

Unno Takuma; Takatsuka Hisashi; Ohnishi Yuto; Ito Masahiro; Kubota Yukihiko

doi:10.1007/s13258-021-01195-9

추천

검색

자료유형: 학술저널

저자정보: Unno Takuma (Ritsumeikan University Japan) Takatsuka Hisashi (Ritsumeikan University Japan) Ohnishi Yuto (Ritsumeikan University Japan) Ito Masahiro (Ritsumeikan University Japan) Kubota Yukihiko (Ritsumeikan University Japan)

저널정보: 한국유전학회 Genes & Genomics Genes & Genomics Vol.44 No.3

발행연도: 2022.3

수록면: 343 - 357 (15page)

DOI: 10.1007/s13258-021-01195-9

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

Background Caenorhabditis elegans encodes three class I histone deacetylases (HDACs), HDA-1, HDA-2, and HDA-3. Although HDA-1 is known to be involved in embryogenesis, the regulatory roles of HDA-2 and HDA-3 in embryonic development remain unexplored. Objective To elucidate the functional roles of the three class I HDACs in C. elegans embryonic development. Methods The roles of Class I HDACs, HDA-1, HDA-2, and HDA-3 in Caenorhabditis elegans during embryogenesis were investigated through the analysis of embryonic lethality via gene knockdown or deletion mutants. Additionally, the size of these knockdown and mutant eggs was observed using a differential interference contrast microscope. Finally, expression pattern and tissue-specific role of hda-2 and transcriptome of the hda-2 mutant were analyzed. Results Here, we report that HDA-1 and HDA-2, but not HDA-3, play essential roles in Caenorhabditis elegans embryonic development. Our observations of the fertilized egg size variance demonstrated that HDA-2 is involved in regulating the size of fertilized eggs. Combined analysis of expression patterns and sheath cell-specific rescue experiments indicated that the transgenerational role of HDA-2 is involved in the viability of embryonic development and fertilized egg size regulation. Furthermore, transcriptome analysis of hda-2 mutant embryos implies that HDA-2 is involved in epigenetic regulation of embryonic biological processes by downregulating and upregulating the gene expression. Conclusion Our finding suggests that HDA-2 regulates the embryonic development in Caenorhabditis elegans by controling a specific subset of genes, and this function might be mediated by transgenerational epigenetic effect.

#C. elegans · HDAC · Epigenomics · Transgeneration · Embryonic lethality · Size regulation

참고문헌 (0)

참고문헌 신청

참고문헌이 DBpia에서 서비스 중이라면, [참고문헌 신청]을 통해 등록해보세요

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!