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자료유형
학술저널
저자정보
저널정보
대한임상검사과학회 대한임상검사과학회지 대한임상검사과학회지 제47권 제4호
발행연도
2015.1
수록면
175 - 181 (7page)

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초록· 키워드

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In order to examine the effect of Smilax china L. (SC) extract on the cytotoxicity of methymercuric chloride (MMC), allergic contact dermatitis, The cytotoxicity of MMC was assessed after cultured NIH3T3 fibroblasts were treated with various concentrations of MMC for 72 hours. And also, the following results were obtained by measuring the antioxidative effect of SC extract on the cytotoxicity of MMC. In this study, MMC remarkably decreased the cell viability of NIH3T3 fibroblasts in a dose-dependent manner, and MMC was seen to be highly-toxic below 100 uM of XTT50 value. In addition, the toxicity of MMC was involved in oxidative stress via a blockage of MMC-induced cytotoxicity by vit. E as antioxidant. In the protective effect of SC extract on MMC-induced cytotoxicity, SC extract defended the cytotoxicity of MMC by a significant increase of cell viability which was decreased by MMC-induced cytotoxicity. It also showed antioxidative effects such as electron donating ability (EDA), superoxide dismutase (SOD)-like activity (SLA) and the lipid peroxidation activity (LPA). From these results, the natural component as SC extract may be a putative resource as the antioxidative agent for the treatment of inflammatory skin disease associated with the oxidative stress.

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