지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Fluorescence lifetime imaging microscopy (FLIM) has been considered an effective technique to investigate chemical properties of the specimens, especially of biological samples. Despite of this advantageous trait, researchers in this f eld have had diff culties applying FLIM to their systems because acquiring an image using FLIM consumes too much time. Although analog mean-delay (AMD) method was introduced to enhance the imaging speed of commonly used FLIM based on time-correlated single photon counting (TCSPC), a real-time image reconstruction using AMD method has not been implemented due to its data processing obstacles. In this paper, we introduce a real-time image restoration of AMD-FLIM through fast parallel data processing by using Threading Building Blocks (TBB; Intel) and octa-core processor (i7-5960x; Intel). Frame rate of 3.8 frames per second was achieved in 1,024×1,024 resolution with over 4 million lifetime determinations per second and measurement error within 10%. This image acquisition speed is 184 times faster than that of single-channel TCSPC and 9.2 times faster than that of 8-channel TCSPC (state-of-art photon counting rate of 80 million counts per second) with the same lifetime accuracy of 10% and the same pixel resolution.
목차
등록된 정보가 없습니다.
참고문헌 (10)
참고문헌 신청
Berezin M Y / 2010 / Fluorescence lifetime measurements and biological imaging / Chemical Reviews 110:2641~2684
Cambridge Technology / 2006 / CRS (Counter Rotation Scanner) User’s Manual / Cambridge Technology
Gilbert D / 2007 / Differential maturation of chloride homeostasis in primary afferent neurons of the somatosensory system / Int. J. Devl. Neuroscience 25:479~489
Hanson K M / 2002 / Two-photon fl uorescence lifetime imaging of the skin stratum corneum pH Gradient / J. Biophysics 83:1682~1690
Kim J / 2015 / Parametric optimization for high speed FLIM implementation / MATEC Web of Conference 32:04011
Cambridge Technology / 2006 / CRS (Counter Rotation Scanner) User’s Manual / Cambridge Technology
Gilbert D / 2007 / Differential maturation of chloride homeostasis in primary afferent neurons of the somatosensory system / Int. J. Devl. Neuroscience 25:479~489
Hanson K M / 2002 / Two-photon fl uorescence lifetime imaging of the skin stratum corneum pH Gradient / J. Biophysics 83:1682~1690
Kim J / 2015 / Parametric optimization for high speed FLIM implementation / MATEC Web of Conference 32:04011
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