지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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Bacteriophages infect host bacteria and control host metabolism through diverse mechanisms. We noted a gene harboring homology in the N-terminal acetyltransferase of a bacteriophage SPN3US, which infects Salmonella. The phage-encoded gene is phylogenetically remote from the typical N-terminal acetyltransferases whose structures are available. This study reports a preliminary analysis of the crystals of phage-encoded N-terminal acetyltransferase, including its purification and crystallization. The crystals diffracted X-rays to a 2.2 A resolution, revealing that the crystals belong to P6122 or P6522 with unit cell parameters of a = 68.5 and c = 219.0 A. We are now growing the selenomethionine-substituted crystals to obtain the phase information. The protein structure will provide molecular insights on how the phage hijacks the bacterial host metabolism.
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참고문헌 (11)
참고문헌 신청
Adams, P. D. / 2010 / PHENIX : a comprehensive Python-based system for macromolecular structure solution / Acta Crystallogr D Biol Crystallogr 66:213~221
Burrowes, B. / 2011 / Bacteriophage therapy : potential uses in the control of antibiotic-resistant pathogens / Expert Rev Anti Infect Ther 9:775~785
Chan, B. K. / 2013 / Phage cocktails and the future of phage therapy / Future Microbiol 8:769~783
Lee, J. H. / 2011 / Complete genome sequence of Salmonella bacteriophage SPN3US / J Virol 85:13470~13471
Mosig, G. / 1998 / Several new bacteriophage T4 genes, mapped by sequencing deletion endpoints between genes 56(dCTPase)and dda(a DNA-dependent ATPasehelicase)modulate transcription / Gene 223:143~155
Burrowes, B. / 2011 / Bacteriophage therapy : potential uses in the control of antibiotic-resistant pathogens / Expert Rev Anti Infect Ther 9:775~785
Chan, B. K. / 2013 / Phage cocktails and the future of phage therapy / Future Microbiol 8:769~783
Lee, J. H. / 2011 / Complete genome sequence of Salmonella bacteriophage SPN3US / J Virol 85:13470~13471
Mosig, G. / 1998 / Several new bacteriophage T4 genes, mapped by sequencing deletion endpoints between genes 56(dCTPase)and dda(a DNA-dependent ATPasehelicase)modulate transcription / Gene 223:143~155
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