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Mycobacterium tuberculosis is a dangerous pathogen, and it can cause the most deadly disease tuberculosis (TB). Nonpathogenic Mycobacterium smegmatis is an important model for studying the M. tuberculosis. M. smegmatis 5’-Methylthioadenosine/S-adenosyl-L-homocysteine nucleosidases (MtaNs) catalyze the hydrolysis of adenine from 5’-methylthioadenosine (MTA), MtaNs cleave the glycosidic bond of MTA or S-adenosylhomocysteine (SAH) irreversibly. In this study, MtaN from M. smegmatis (MsMtaN) was successfully expressed and purified using Ni-NTA affinity, Q anionexchange and gel-filtration chromatography. The protein crystal was obtained and diffracted to a resolution of 2.0 Å. The crystal belonged to the orthorhombic space group P1211, with unit-cell parameters of a =57.6, b = 172.6, and c = 183.3 Å. The Matthews coefficient and solvent content were estimated to be 2.32 Å3 Da-1 and 47%, respectively, assuming that the asymmetric unit contained only one recombinant protein molecule. Size-exclusion chromatography suggested that MsMtaN prefer to exist as tetramer in solution.
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참고문헌 (29)
참고문헌 신청
Alajmi, M. F. / 2018 / Antimycobacterial Activity and Safety Profile Assessment of Alpinia galanga and Tinospora cordifolia / Evid Based Complement Alternat Med 2018:2934583
Bhalla, A. S. / 2015 / Chest tuberculosis : Radiological review and imaging recommendations / Indian J Radiol Imaging 25:213~225
Bohsali, A. / 2010 / The nonpathogenic mycobacteria M. smegmatis and M. fortuitum induce rapid host cell apoptosis via a caspase-3 and TNF dependent pathway / BMC Microbiol 10:237
Chen, J. / 2019 / Crystal Structure of Aeromonas hydrophila Cytoplasmic 5'-Methylthioadenosine/S-Adenosylhomocysteine Nucleosidase / Biochemistry 58:3136~3143
Cornell, K. A. / 2009 / Assessment of methylthioadenosine/S-adenosylhomocysteine nucleosidases of Borrelia burgdorferi as targets for novel antimicrobials using a novel high-throughput method / J Antimicrob Chemother 63:1163~1172
Bhalla, A. S. / 2015 / Chest tuberculosis : Radiological review and imaging recommendations / Indian J Radiol Imaging 25:213~225
Bohsali, A. / 2010 / The nonpathogenic mycobacteria M. smegmatis and M. fortuitum induce rapid host cell apoptosis via a caspase-3 and TNF dependent pathway / BMC Microbiol 10:237
Chen, J. / 2019 / Crystal Structure of Aeromonas hydrophila Cytoplasmic 5'-Methylthioadenosine/S-Adenosylhomocysteine Nucleosidase / Biochemistry 58:3136~3143
Cornell, K. A. / 2009 / Assessment of methylthioadenosine/S-adenosylhomocysteine nucleosidases of Borrelia burgdorferi as targets for novel antimicrobials using a novel high-throughput method / J Antimicrob Chemother 63:1163~1172
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