지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2024.3
- 수록면
- 8 - 12 (5page)
- DOI
- 10.34184/kssb.2024.12.1.8
이용수
초록· 키워드
오류제보하기
Bak is the proapoptotic Bcl-2 protein family member, forming an oligomerized pore at the mitochondrial outer membrane for the release of cytochrome c from the mitochondria into the cytosol. Due to its apoptosis-triggering role, Bak is considered a potential therapeutic drug target. Notably, eltrombopag, an FDA-approved thrombopoietin receptor agonist, was identified as a novel Bak-binding proapoptotic molecule. In this study, the recombinant Bak protein produced in Escherichia coli was purified and then mixed with eltrombopag at a 1:1.2 molar ratio. Crystals were obtained using the sample, and utilized to collect X-ray diffraction data with a maximum resolution of 1.40 Å. Preliminary diffraction analysis indicated that our crystals belonged to the P63 space group with unit cell parameters a = b = 73.5 Å and c = 44.6 Å. In a single asymmetric unit, one protein molecule is present, with a 36.5% solvent content and a 1.94 Å3/Da Matthews coefficient.
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