지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
이용수
초록· 키워드
오류제보하기
Periplasmic terephthalic acid binding protein (TBP) from Ideonella sakaiensis (IsTBP) is a periplasmic protein that imports TPA to the cytoplasm interacting with the TTT-transport system. In this study, we overexpressed IsTBP and purified the protein to homogeneity by Ni-NTA affinity and size-exclusion chromatography. The IsTBP protein was crystallized using hanging-drop vapor-diffusion method in the presence of 20% PEG3350 and 0.2 M Ammonium iodide at 20°C. X-ray diffraction data were collected to a maximum resolution of 1.02 Å. The IsTBP crystals belonged to the space group P21 with unit cell parameters a = 46.2 Å, b = 54.1 Å, c = 50.6 Å. With one molecule of IsTBP per asymmetric unit, the crystal volume per unit of protein mass was 2.32 Å3 Da–1, which corresponds to a solvent content was approximately 47.00%.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Purification, Crystallization and X-ray crystallographic analysis of Homoserine O-succinyltransferase from Escherichia coli
Biodesign
2019 .01
-
Crystallization and preliminary diffraction analysis of Bak incubated with eltrombopag
Biodesign
2024 .03
-
Expression, purification and crystallization of GSK3β in complex with the flavonoid morin
Biodesign
2018 .01
-
Purification, crystallization, and X-ray crystallographic analysis of ribulose bisphosphate carboxylase large subunit from Hydrogenovibrio marinus
Biodesign
2024 .09
-
Purification, crystallization and X-ray crystallographic analysis of cystathionine gamma-synthase from Corynebacterium glutamicum
Biodesign
2017 .03
-
반강자성 IrMn 층과 인접한 Co/Ni 다층박막의 수직 교환 바이어스
한국자기학회지
2017 .12
-
Preliminary crystallographic analysis of the Fab fragment of camrelizumab, a therapeutic antibody against PD-1
Biodesign
2019 .01
-
Purification, crystallization and X-ray crystallographic analysis of glycine oxidase from Bacillus cereus ATCC 14579
Biodesign
2020 .01
-
Crystallization and preliminary diffraction analysis of human TEAD1, a transcriptional enhancer factor that controls the Hippo signaling pathway
Biodesign
2018 .01
-
A novel nonsense mutation in NPR2 gene causing Acromesomelic dysplasia, type Maroteaux in a consanguineous family in Southern Punjab (Pakistan)
Genes & Genomics
2020 .01
-
Cytosolic 5′-Methylthioadenosine/ S-Adenosylhomocysteine Nucleosidase MtaN from Mycobacterium. smegmatis: purification, crystallization and X-ray crystallographic analysis
Biodesign
2019 .01
-
Aeromonas hydrophila cytosolic 5’-methylthioadenosine/S-adenosylhomocysteine nucleosidase MtaN-2: crystallization and X-ray crystallographic analysis
Biodesign
2018 .01
-
Purification, crystallization, and X-ray crystallographic analysis of histidine triad nucleotide-binding protein from Candida albicans
Biodesign
2018 .01
-
Bacillus amyloliquefaciens NAD+-dependent protein deacetylase: purification, crystallization and X-ray crystallographic analysis
Biodesign
2019 .01
-
Purification, crystallization and X-ray crystallographic analysis of mesodiaminopimelic acid decarboxylase from Corynebacterium glutamicum
Biodesign
2017 .06
-
IrMn 층이 인접한 Co/Ni 다층박막에서의 비대칭 자화역전현상 연구
한국자기학회지
2025 .02
-
Purification, crystallization and X-ray crystallographic analysis of methylmalonyl- CoA epimerase from Metallosphaera sedula
Biodesign
2021 .01
-
Overexpression, crystallization, and preliminary X-ray diffraction studies of NDUFA2
Biodesign
2023 .06
-
Purification, crystallization and preliminary X-ray crystallographic analysis of VCA0593 with a c-di-GMP binding activity in Vibrio cholerae
Biodesign
2017 .06
-
Purification, crystallization and X-ray crystallographic analysis of enoyl-CoA hydratase/isomerase-family protein from Cupriavidus necator H16
Biodesign
2018 .06
이 논문의 저자 정보
최근 본 자료
댓글(0)
0